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| Id: | 23200
| | Autor: | Kramme, Stefanie; Bretzel, Gisela; Panning, Marcus; Kawuma, Joseph; Drosten, Christian
| | Título: | Detection and quantification of Mycobacterium leprae in tissue samples by real-time PCR
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| | Fonte: | s.l; s.n; 2004. 5 p. tab, graf.
| | Resumo: | Real-time PCR technology has improved molecular diagnostics of many pathogens, but no such test is available for Mycobacterium leprae. In this report we describe the establishment and the pre-clinical evaluation of such an assay. The test achieved a theoretical analytical sensitivity limit of 194 M. leprae cells per skin biopsy specimen and facilitated quantification of mycobacteria in tissue over a range of 54-54,000,000 cells per sample. In punch skin biopsies from 39 untreated Ugandan patients with newly diagnosed leprosy, the clinical diagnosis could be confirmed in 88.9% of multibacillary and 33.3% of paucibacillary (microscopically negative) patients. Real-time detection thus did not increase the clinical sensitivity of PCR as compared to conventional protocols, in spite of its evidently high analytical sensitivity. On the other hand, as still no culture system exists for M. leprae, the assay appears to be a robust tool for detection of the bacterium in selected clinical situations, as well as for quantitation in experimental settings. (AU).
| | Descritores: | Antígenos de Bactérias/GE
Proteínas de Bactérias/GE
Biópsia
DNA Bacteriano/CH/IP
Hanseníase/*DI/MI
Dados de Sequência Molecular
Mycobacterium leprae/GE/*IP
Reação em Cadeia da Polimerase/*MT
Sensibilidade e Especificidade
Alinhamento de Sequência
Análise de Sequência de DNA
Pele/MI
Uganda
| | Limites: | Research Support, Non-U.S. Gov't
Humanos
| | Localização: | BR191.1; 09344/s |
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